There are twenty six unlockable bonus costumes in Mario Tennis Open for the Nintendo 3DS.
Here are the bonus costumes, and how to unlock them.
Bee Mario – Turn eight characters into star characters
Birdo – Defeat an Ace opponent in a singles Exhibition match
Boo – Purchase 100 different items in the item shop
Bowser Jr – Get 20 Victory Medals from Online Multiplayer Open Matches
Bowser – Get 50 Victory Medals from Online Multiplayer Open Matches
Cloud Mario – Turn 10 characters into star characters
Daisy – Collect 700 ring points in Ring Shot Callenge with another player
Diddy Kong – Meet 20 Mii characters via StreetPass
Donkey Kong – Meet 50 Mii characters via StreetPass
Dry Bowser – Get 100 Victory Medals from Online Multiplayer Open Matches
Fire Mario – Turn two characters into star characters
Goomba – Add 100 items to the item shop shelves
Koopa Troopa – Win 10 consecutive StreetPass matches
Luigi – Win the Star Final Cup Tournament in Doubles
Luma – Collect 1,500 coins in the Galaxy Rally Challenge Special Game
Mario – Win the Star Final Cup Tournament in Singles
Peach – Collect 800 ring points in Ring Shot Callenge
Petey Piranha – Get 50 returns in the Ink Showdown Challenge
Propeller Mario – Turn five characters into star characters
Shy Guy – Win 20 consecutive StreetPass matches
Tanooki Mario – Turn 15 characters into star characters
Toad – Collect a total of 50,000 coins
Waluigi – Collect 800 Coins in Super Mario Tennis Level 2- 4
Wario – Collect 800 Coins in Super Mario Tennis Level 1- 4
Wiggler – Get a total of 5,000 points in Online Multiplayer Open matches
Yoshi – Scan the QR Code from the official Nintendo website